Journal: Journal of Cellular Physiology

Article Title: Silica‐coated magnetic nanoparticles labeled endothelial progenitor cells alleviate ischemic myocardial injury and improve long‐term cardiac function with magnetic field guidance in rats with myocardial infarction

doi: 10.1002/jcp.28492

Figure Lengend Snippet: (a) Representative images and bar graphs of the transmigrated cell number in the labeled and unlabeled endothelial progenitor cell (EPC) groups (×200). (b) Proliferation curve of labeled and unlabeled EPCs in the successive five days. (c) Representative images of tube formation capacities in the labeled and unlabeled EPC groups (×200). The bar graphs show that there was no statistical difference in total tube length and total vascular lumen number between the two groups ( p > 0.05). (d) Representative histograms of the cell phenotype of labeled and unlabeled EPCs in the FACS analysis. (e) Western blot analysis of the expression of VEGF, IGF‐1, FDGF, SDF‐1α, and β‐FGF in the labeled and unlabeled EPC groups. (f) Plasma levels of cTnI when transplanted with different doses of EPCs, and values were expressed as mean ± standard deviation ( SD ). * p < 0.05 versus the other group. cTnI: cardiac troponin I; FACS: fluorescence‐activated cell sorting [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Then, 5% nonfat dried milk were used to block the nonspecific binding sites in the membranes, and the membranes were incubated with the primary antibodies of VEGF (BS6496; Bioworld, MN), IGF‐1 (BA0939; Boster, Wuhan, China), FDGF (Ab16829; Abcam, Cambridge, British), SDF‐1α (E‐AB‐32858; Elabscience, Wuhan, China), β‐FGF (bs‐0217R; Bioss, Beijing, China), ANP (DF6479; Affinity), BNP (Ab19465; Abcam), Bax (Ab32503; Abcam), and Bcl‐2 (Ab32124; Abcam) overnight, followed by incubation with the corresponding secondary antibodies.

Techniques: Labeling, Western Blot, Expressing, Standard Deviation, Fluorescence, FACS

Journal: Journal of Cellular Physiology

Article Title: Silica‐coated magnetic nanoparticles labeled endothelial progenitor cells alleviate ischemic myocardial injury and improve long‐term cardiac function with magnetic field guidance in rats with myocardial infarction

doi: 10.1002/jcp.28492

Figure Lengend Snippet: (a) Representative Masson staining images of the heart papillary muscle cross‐sectional scan images, and infarct rate of the different groups. (b) Upper panel: Bar graphs of LVEF and LVFS before and at 4 weeks after the transplantation of EPCs in the different groups. Lower panel: Western blots analysis of the expression of ANP and BNP in the different groups. (c) Western blot analysis of the expression of VEGF, IGF‐1, FDGF, SDF‐1α, and β‐FGF in the different groups. (d) Representative images of CD31 and GFP labeled EPC double staining (×200), and the bar graph of the cell number of GFP + and CD31 + in the different groups. CD31 labeled capillary density (red); GFP labeled EPCs (green). The values were expressed as mean ± standard deviation ( SD ). * p < 0.05 versus the SO group; ** p < 0.05 versus the MI group; # p < 0.05 versus the MI + Fe‐EPC group; ## p < 0.05 versus the other group. EPC: endothelial progenitor cell; GFP: green fluorescent protein; LVEF: left ventricular ejection fraction; LVFS: left ventricular fractional shortening; MI: myocardial infarction; SO: sham‐operated [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Then, 5% nonfat dried milk were used to block the nonspecific binding sites in the membranes, and the membranes were incubated with the primary antibodies of VEGF (BS6496; Bioworld, MN), IGF‐1 (BA0939; Boster, Wuhan, China), FDGF (Ab16829; Abcam, Cambridge, British), SDF‐1α (E‐AB‐32858; Elabscience, Wuhan, China), β‐FGF (bs‐0217R; Bioss, Beijing, China), ANP (DF6479; Affinity), BNP (Ab19465; Abcam), Bax (Ab32503; Abcam), and Bcl‐2 (Ab32124; Abcam) overnight, followed by incubation with the corresponding secondary antibodies.

Techniques: Staining, Transplantation Assay, Western Blot, Expressing, Labeling, Double Staining, Standard Deviation

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Melanoma cell-secreted exosomal miR-155-5p induce proangiogenic switch of cancer-associated fibroblasts via SOCS1/JAK2/STAT3 signaling pathway

doi: 10.1186/s13046-018-0911-3

Figure Lengend Snippet: Melanoma cell-secreted exosomes reprogram fibroblasts into proangiogenic CAFs in vitro and in vivo. Treatment with B16- and B16F10-secreted exosomes for 24 h significantly upregulated the expressions of FGF2, VEGFa, and MMP9 in NIH/3T3 cells as shown by RT-PCR ( a ) and Western blot ( b ) (The ratio of the expressions of MMP9, VEGFa, and FGF2 is indicated below the panels), and promoted the secretion of these proangiogenic factors as shown by ELISA ( c ). Independent experiments performed in triplicate. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control group. d , e , f , and g CCK-8 assay, transwell migration assay, and tube formation assay showed that the CM from NIH/3T3 cells treated with B16- and B16F10-secreted exosomes promoted MS-1 cell proliferation, migration, and tube formation. Independent experiments performed in triplicate. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control group. Scale bar, 50 μm. One-way ANOVA and student’s t-tests. h Representative photographs of Matrigel plugs harvested at 1 week after subcutaneous injection into C57BL/6 mice ( n = 5). i Representative immunofluorescence images of the neovessels in the Matrigel plugs. Vasculature and nuclear staining were performed by using CD31 (red) and DAPI (blue), respectively. Scale bar, 50 μm. j Quantitative analysis of the neovessels in the Matrigel plugs . * P < 0.05, ** P < 0.01, student’s t-tests. Values are represented as means ± SEM. CAFs: cancer-associated fibroblasts. CCK-8: Cell Counting Kit-8. CM: conditioned medium. DAPI: 4′6-diamidino-2-phenylindole. ELISA: Enzyme-linked immunosorbent assay. Exo: exosomes. FGF2: fibroblast growth factor 2. GAPDH: Glyceraldehyde 3-phosphate dehydrogenase. MMP9: matrix metalloproteinase 9. SEM: standard error of the mean. VEGFa: vascular endothelial growth factor A

Article Snippet: FGF2 antibody (Cat.No. sc-136255) was purchased from Santa Cruz.

Techniques: In Vitro, In Vivo, Reverse Transcription Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay, Control, CCK-8 Assay, Transwell Migration Assay, Tube Formation Assay, Migration, Injection, Immunofluorescence, Staining, Cell Counting

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Melanoma cell-secreted exosomal miR-155-5p induce proangiogenic switch of cancer-associated fibroblasts via SOCS1/JAK2/STAT3 signaling pathway

doi: 10.1186/s13046-018-0911-3

Figure Lengend Snippet: Melanoma cell-secreted exosomes regulate the proangiogenic switch of CAFs via SOCS1/JAK2/STAT3 signaling pathway. a Treatment with B16- and B16F10-secreted exosomes elevated the phosphorylation levels of JAK2 and STAT3 and suppressed the expression of SOCS1. The ratio of the expression of SOCS1 and the phosphorylation levels of JAK2 and STAT3 are indicated below the panels. b Western blotting of nuclear P-STAT3 expression. The nuclear protein Histone H3 was used as the nuclear protein marker. c Treatment with AG490 downregulated the mRNA expressions of VEGFa, MMP9, and FGF2 in CAFs. * P < 0.05, ** P < 0.01, *** P < 0.001. One-way ANOVA and student’s t-tests. d , e Treatment with AG490 downregulated the phosphorylation levels of JAK2 and STAT3, and the expressions of VEGFa, MMP9, and FGF2 in CAFs. f , g The promotive effect of the CM from NIH/3T3 cells treated with B16- and B16F10-secreted exosomes on MS-1 cell proliferation was blocked by AG490. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. the CM from NIH/3T3 cells; # P < 0.05, ## P < 0.01 vs. the CM from NIH/3T3 cells treated with B16- and B16F10-secreted exosomes. h , i , and j The promotive effects of the CM from NIH/3T3 cells treated with B16- and B16F10-secreted exosomes on MS-1 cell migration and tube formation were blocked by AG490. * P < 0.05, ** P < 0.01. Independent experiments performed in triplicate. Values are expressed as means ± SEM. One-way ANOVA and student’s t-tests. Scale bar, 50 μm. CAFs: cancer-associated fibroblasts. CM: conditioned medium. Exo: exosomes. FGF2: fibroblast growth factor 2. GAPDH: Glyceraldehyde 3-phosphate dehydrogenase. JAK2: Janus kinase 2. MMP9: matrix metalloproteinase 9. SEM: standard error of the mean. SOCS1: suppressor of cytokine signaling 1. STAT3: signal transducer and activator of transcription 3. VEGFa: vascular endothelial growth factor A

Article Snippet: FGF2 antibody (Cat.No. sc-136255) was purchased from Santa Cruz.

Techniques: Phospho-proteomics, Expressing, Western Blot, Marker, Migration

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Melanoma cell-secreted exosomal miR-155-5p induce proangiogenic switch of cancer-associated fibroblasts via SOCS1/JAK2/STAT3 signaling pathway

doi: 10.1186/s13046-018-0911-3

Figure Lengend Snippet: Exosomal miR-155 regulates the proangiogenic switch of CAFs in vitro. a , b Western blot and densitometry analysis showed that treatment with exosomes from miR-155-mimic-transfected B16 cells downregulated the expression of SOCS1, elevated the phosphorylation levels of JAK2 and STAT3, and upregulated the expressions of MMP9, VEGFa, and FGF2. Whereas treatment with exosomes from anti-miR-155-transfected B16F10 cells resulted in the opposite effects. The ratios of the expressions of SOCS1, MMP9, VEGFa, and FGF2, and the phosphorylation levels of JAK2 and STAT3 are indicated below the panels. c , d CCK-8 assay showed that the CM from NIH/3T3 cells treated with exosomes extracted from miR-155-mimic-transfected B16 cells promoted MS-1 cell proliferation. Whereas the CM from NIH/3T3 cells treated with exosomes extracted from anti-miR-155-transfected B16F10 cells had the opposite effect. * P < 0.05, ** P < 0.01 vs. the CM from nontreated NIH/3T3 cells. # P < 0.05, ## P < 0.01 vs. the miR-NC-transfected group or anti-NC-transfected group. Student’s t-tests. e , f , and g) Overexpression of miR-155 in exosomes enhanced the promotive effects of the CM from NIH/3T3 cells that treated with miR-155-mimic-transfected B16-secreted exosomes on MS-1 cell proliferation and tube formation. Whereas reduction of miR-155 in exosomes resulted in the opposite effects ( h , i , and j ). * P < 0.05, ** P < 0.01, *** P < 0.001. Independent experiments performed in triplicate. Values are expressed as means ± SEM. Student’s t-tests. Scale bar, 50 μm. Anti-NC: inhibitor negative control. Anti-miR-155: miR-155 inhibitor. CAFs: cancer-associated fibroblasts. CM: conditioned medium. Exo: exosomes. FGF2: fibroblast growth factor 2. GAPDH: Glyceraldehyde 3-phosphate dehydrogenase. JAK2: Janus kinase 2. MiR-NC: miR-negative control. MMP9: matrix metalloproteinase 9. SEM: standard error of the mean. SOCS1: suppressor of cytokine signaling 1. STAT3: signal transducer and activator of transcription 3. VEGFa: vascular endothelial growth factor A

Article Snippet: FGF2 antibody (Cat.No. sc-136255) was purchased from Santa Cruz.

Techniques: In Vitro, Western Blot, Transfection, Expressing, Phospho-proteomics, CCK-8 Assay, Over Expression, Negative Control

Journal: Anatolian Journal of Botany

Article Title: Protective effects of resveratrol on permethrin-induced fetotoxicity in rats

doi: 10.30616/ajb.1241886

Figure Lengend Snippet: Figure 2. Representative photomicrographs of immuno- histochemical staining of fetal tissues. Immunohistochemical staining for BMP-4 in fetal bone and FGF-1 in fetal lung. In the Perm group, deterioration in the structure of the lacunae and

Article Snippet: In experiments, Perm (Permethrin, 45614, Sigma), Res (Resveratrol, 51852282, Molecula), BMP-4 primary antibody (PA5-19683, Thermo Scientific), FGF-1 primary antibody (sc-7910, Santa Cruz), and Secondary antibody kit (Thermo, Cat. No: 85-9043) were used.

Techniques: Staining, Immunohistochemical staining

Journal: Cell Transplantation

Article Title: Locally Administered Adipose-Derived Stem Cells Accelerate Wound Healing through Differentiation and Vasculogenesis

doi: 10.3727/096368910x520065

Figure Lengend Snippet: Figure 7. Effects of ASCs on VEGF, HGF, and FGF2 levels. (A–C) The results of ELISA showed that significantly increased levels of VEGF and HGF secreted by ASCs were observed after 72-h culture. The FGF2 concentration was maintained at a relatively low level. (D–F) The protein expression of VEGF, HGF, and FGF2 in wound was analyzed by Western blot. Protein levels of VEGF and HGF were significantly elevated in ASC-treated wounds compared with control wounds. There was no signifi- cant difference of protein expression between the control group and fibroblast groups. A slight but statistically significant enhance- ment in FGF2 protein expression was detected in the ASC-treated group. *p < 0.05 versus control group.

Article Snippet: After blotting, the membranes were incu- bated with primary antibodies against VEGF (1:1000, also negative for the integrin CD11, an adhesion molecule characteristically found on leukocytes (Fig. 1A).ab70612, Abcam), HGF (1:1000, ab83760, Abcam), FGF2 (1:500, sc-7911, Santa Cruz), and β-actin (1:1000, The adherent cultured ASCs exhibited a fibroblast-like morphology (Fig. 1B).

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Expressing, Western Blot, Control